Problem with Kasumi-6 cell line (View forum version)



Elfka

Posted 29 March 2013 - 06:50 PM

Hi all,

I am facing problems in growing Kasumi-6 cell line. I'm using RPMI with 20% FBS and then directly do the flask I'm adding 2ng/ml GMCSF. But they not growing I don’t know where is the problem.
Can anybody pls suggest any protocol for culture method for Kasumi-6 cells so that I can get healthy proliferating cells for my further work


Any suggestions will be highly apprciated.

Thanks

GaryJing

Posted 07 July 2013 - 07:13 AM

Hi, besides the FBS and GMCSF, do you put the following components into the cultue medium: 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate?
One more thing, please maintain the cell density between 2 x 105 and 1.5 x 106 viable cells/mL.