Controls for co-immunoprecipitation (View forum version)



Lfs

Posted 25 March 2013 - 03:00 PM

Hi!

I gonna start to work with co-IP (Pierce), but I never did this. I was reading about it, but it seems so confusing! I'm working with 2 cytoplasmatic proteins and co-transfecting using HEK 293. Which is the best positive and negative control in this case?

Thank you very much!!!

bob1

Posted 26 March 2013 - 12:38 AM

Controls could be no antibody, non-specific (i.e. not one you expect to show interaction), serum from the animal(s) the antibodies were raised in, transfected and untransfected cells...

Lfs

Posted 26 March 2013 - 09:25 AM

Thanks, Bob!

HOYAJM

Posted 27 March 2013 - 12:33 PM

Just to add one more, if you have these genes knocked out in a particular genotype, antibody treatment with these make for great negative controls. I also believe for this type of work, knockdown controls are also useful with siRNA