lymphocytes culture (View forum version)



sagni2002

Posted 23 April 2012 - 07:31 AM

Hello everyone,

by lack of money in my laboratory we have no incubator CO2.and my question is: can we do in a lymphocytes culture in incubator without CO2.

Thank you.

cell slave

Posted 25 April 2012 - 05:04 AM

Hello, sagni2002. I used to culture lymphocytes in sealed sterile (30ml) universal tubes (in case you have a problem with culture flasks as well), without CO2.

rhombus

Posted 25 April 2012 - 07:54 AM

Hello, sagni2002. I used to culture lymphocytes in sealed sterile (30ml) universal tubes (in case you have a problem with culture flasks as well), without CO2.



Cell slave is correct, you can grow all cells without CO2 in a selaed tissue culture flask. However you must use a media that has 25mM HEPES buffer AND refed constantly to replenish oxygen

Kindest regards

Uncle Rhombus

sagni2002

Posted 04 May 2012 - 10:17 AM

hello,
is what it is like if I make my cultures in microplates.

Tlank you.