Denaturing agent Acrylamide-bisacruylamide gel (View forum version)



vincio

Posted 28 March 2012 - 11:52 PM

Hi,
what are the the denaturing agents for poliacrylamide sequencing gels different from Urea and formamide? I am asking for the same used in reprogel because has a good shelf life but there are not information a respect
Thank you

mdfenko

Posted 29 March 2012 - 06:17 AM

looking at the msds for reprogel (solution B), the only chemicals that are unusual are 2-pyrrolidinone (aka 2-pyrrolidone) and ethanediol (aka ethylene glycol).

keep in mind that the formulation of reprogel is proprietary and patented.

phage434

Posted 29 March 2012 - 11:02 AM

If it is patented, then you can find out exactly what is in it and how to make it. This is quite different from what is normally called "proprietary" which usually means "it's a secret and I won't tell you."

mdfenko

Posted 30 March 2012 - 08:11 AM

you're right, probably not proprietary, especially since the msds tells the percentage of each component in the stock buffer.

mdfenko

Posted 30 March 2012 - 08:37 AM

if you don't tell anybody...oops

vincio

Posted 30 March 2012 - 09:47 AM

...tell what??

vincio

Posted 30 March 2012 - 09:50 AM

I will continue buyng this product...but it is quite expensive...45 euro each 75 ml of ready to use solution!!!...1 liter of Acrylamide bis acrylamide cost 30 euro!!!!