Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody (View forum version)



sansub

Posted 12 January 2012 - 03:56 PM

The western blot was done for CHO, COS-7 and NIH3T3 cell lysates which were also treated with DFP ( a protease inhibitor) using the insulin receptor beta antibody (C-19, purchased from Santa Cruz). I obtained the required band at 95kDa confirming the presence of the insulin receptor beta subunit in these lysates. But, why do I see an extra band at around 60 kDa also? What could be the reason behind that?
4. IR blot+DFP.png
Please help!

mdfenko

Posted 13 January 2012 - 06:37 AM

that (those) extra band(s) are most likely an artifact (previously identified to be keratins) caused by old reducing agent and dust.