Sonicator for ChIP (View forum version)



darshini

Posted 25 November 2011 - 03:48 AM

Hello,

I have to perform ChIP for DNA shearing and the volume size of my samples is really small about 0.2ml - 0.5ml. I am planning on getting a Branson sonicator but I can't decide on the output power and probe size. Can someone please tell me what should be the ideal probe size if I intend to carry out sonication in 1.5ml MCT and if 150 watts output power is sufficient or not?

Thanks,
Darshini

PAO_ahac

Posted 02 December 2011 - 06:19 AM

http://www.sonicator.com/

dedee

Posted 05 December 2011 - 10:00 AM

We're using a bioruptor from diagenode. I love that thing! It's not a probe sonicator, so no issues with bubbles, unevenly cooled samples etc.
I cannot recomment the flow cooler though - we got uneven sonication with this. Just change the ice in the water bath regularly.

PAO_ahac

Posted 05 December 2011 - 10:29 AM

You can use the q sonicator which has options for tips or use in water/ice bath.