How to extract total protein from gram negative bacteria for SDS page and BCA an (View forum version)



tami84

Posted 19 September 2011 - 01:00 PM

Hi everyone,

I'm working with gram negative bacteria (Salmonella), I need to extract total cell protein for SDS page, BCA quantification, and ms analysis.
Maybe someone could share proper protocol,

any help is appreciated

tami

Kamran

Posted 19 September 2011 - 08:40 PM

Please go through these article;

http://onlinelibrary...t.200800024/pdf

http://www.springerl...j5/fulltext.pdf

tami84

Posted 20 September 2011 - 12:37 AM

Thank you, seems to be usefull articles, but they discuss soluble proteins only, i want to extract both soluble and insoluble proteins. Maybe you can reffer me to some other articles that you know. Everything I find on internet wasn't really usefull, i don't have too much reagents in my lab, searching for some simple protocol.
Thanks for your help

tami