Autoclave before adding MgCl2 and MgSO4? (View forum version)



Marvin

Posted 06 April 2011 - 07:06 AM

Hi,

I have a protocol with a SOB receipt that said autoclave the solution before adding MgCl2 and MgSO4. I just wondering if autoclave will destroy these two compounds, or is there any reason for this?

Also, it is necessary to set the pH to 7?

Thanks.

pito

Posted 06 April 2011 - 07:33 AM

Its not that you will destroy them, because both are soluble in water and you can autoclave them without problems.


But why you cant put them in before autoclaving? Good question, I think it has to do with other compononts in the medium that react with MgCl2 and MgSO4 and form precipitation or ...

But the detais about it: no idea...

Maybe you should autoclave a small sample with them added prior to autoclaving to see the difference?

Marvin

Posted 06 April 2011 - 07:40 AM

Its not that you will destroy them, because both are soluble in water and you can autoclave them without problems.


But why you cant put them in before autoclaving? Good question, I think it has to do with other compononts in the medium that react with MgCl2 and MgSO4 and form precipitation or ...

But the detais about it: no idea...

Maybe you should autoclave a small sample with them added prior to autoclaving to see the difference?


Thanks. I do find some protocol that autoclave all compounds together. Maybe I'll just try it out sometime :)

HomeBrew

Posted 06 April 2011 - 12:04 PM

I've always filter sterilized my Mg2+ stock, and added it aseptically to the autoclaved SOB...

philman

Posted 07 April 2011 - 06:46 AM

I autoclaved my SOB with the Mg salts in and it seemed to work fine, the only one I seem to remember where you have to be careful is if you are making SOC and adding glucose.