DNA isolation (View forum version)



pavi

Posted 10 January 2011 - 06:46 PM

Does the concentration of alcohol added affect the amount of DNA that precipitates out?

Ameya P

Posted 10 January 2011 - 09:30 PM

Does the concentration of alcohol added affect the amount of DNA that precipitates out?


It depends on what concentration is your alcohol at? Say, if you added 60% or 80% alcohol instead of 70% alcohol, it would not make a huge difference, but if you added like 20% alcohol, then the DNA that would precipitate out would be very little because it would dissolve in the water.

ElHo

Posted 12 January 2011 - 03:23 AM

The most important thing for ethanol precipitation is to end up with a final ethanol concentration of 70%. This is usually achieved by adding 2.5 volumes of 100% ethanol to the DNA solution you want to precipitate.