cloning of a 20 kb insert (View forum version)



Sank

Posted 08 September 2010 - 10:34 AM

Hello Everyone
I want to clone a 20 kb upstream region of a gene into a vector.
I have never cloned such a long fragment before.
Can anyone suggest some vectors which can hold very long inserts.

Thanks
Sankella

phage434

Posted 08 September 2010 - 04:41 PM

pFOS1 or pBelobac11, both based on the low copy F plasmid origin, will easily hold 20 Kb inserts.

Sank

Posted 09 September 2010 - 05:48 AM

Thanks Phage 434