is it ok to use EtOH or MeOH instead of Butanol? (View forum version)


Posted 15 June 2010 - 11:10 PM

I just ran out of butanol and only have ethanol and methanol in the lab. is it ok to use those to make a smooth surface for lower gel?


Posted 16 June 2010 - 12:58 AM

Yes, it is.
In my lab we do it always with ethanol.


Posted 16 June 2010 - 06:12 AM

Chakchel, do you have phase interface between ethanol and buffer/acrylamide solution?

In my lab butanol is usually "borrowed" by people who use it for TLC so we switched to amyl alcohol. :lol:


Posted 16 June 2010 - 07:12 AM

When EtOH is pipetted too fast onto the freshly poured gel, you may see a slight interphase, but after a short time the EtOH is only one phase above the polymerizing gel.
We never had any problems with EtOH.
Some groups I've been at also use water.