How to prepare ascorbic acids (View forum version)



ryu-a

Posted 04 June 2010 - 02:22 AM

Hello, can somebody tell me how to prepare 25μg/ml ascorbic acid? What should I dissolve it in?

Gerard

Posted 04 June 2010 - 04:30 AM

Hello, can somebody tell me how to prepare 25μg/ml ascorbic acid? What should I dissolve it in?


Very little information, but most of the time you will dissolve it in water short before you use in the test because it will rappidly be oxidized by air

ryu-a

Posted 06 June 2010 - 06:49 AM

Hello, can somebody tell me how to prepare 25μg/ml ascorbic acid? What should I dissolve it in?


Very little information, but most of the time you will dissolve it in water short before you use in the test because it will rappidly be oxidized by air


Thanks for your reply. I wanted to use the ascorbic acid to make 20% FBS medium (DMEM + FBS + ascorbic acid). I was wondering because if I dissolve it in water, the basal DMEM would be diluted and the concentration will change. Is it OK?

Gerard

Posted 06 June 2010 - 08:02 AM

It's my my field of work but if i'm correct DMEM is a water based solution then dissolve the ascorbic acid directly into the medium.

DavidJ

Posted 07 June 2010 - 01:44 AM

We dissolve in DMEM. It may depend on which form you have, but the active form is very light sensitive. We do store them in dark eppendorfs once dissolved at -20. Do not refreeze. Hope it helps