Serum substitutes (View forum version)



SYC1234

Posted 16 November 2009 - 01:08 PM

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! :) !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia

bob1

Posted 16 November 2009 - 04:12 PM

Invitrogen is starting to produce serum free culture systems, you could try them.

klinmed

Posted 17 November 2009 - 09:15 AM

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! ;) !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia

Fetal calf serum (FCS) and fetal bovine serum (FBS) are different names for the same product! It is obtained by cardiac-puncture of bovine fetuses (cow fetus=unborn calf) following slaughter of the cow.
Not a very nice product, but there you are.

pesji

Posted 18 November 2009 - 05:27 AM

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! :) !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia


Hi Sonia,

If you want to go away from FBS you can try Primatone extract which for certain cells works good (certain hybridoma lines)