Immunofluorescence (View forum version)


Posted 23 October 2009 - 05:36 PM


Im going to do an immunofluorescence staining in my samples. However the amount of antibody that I shoul pick is too small. I was thinking in get a reasonable amount and dilute in blocking buffer. What I did not use, store at 4C. Anyone knows if I can store antibody in blocking buffer like this for a week?!



Posted 24 October 2009 - 09:50 PM

Sometimes you can, if the antibody is good. 3-4 days should be fine. However add sodium azide to protect it from contamination.

Good luck


Posted 13 November 2009 - 07:04 AM

i have done it with my antibody and it works fine for me.....all the best


Posted 13 November 2009 - 07:55 AM

It depends on the antibody.
You can even store it at -20C, this way you should be able to keep it longer. I did it with some antibodies I wasn't using too often and it worked just fine.