I agree with Thelymitra pulchella. Your protein may be aggregating into inclusion body form. For avoiding this situation, you may need some advises, such as 1) fusion of the target protein with a more soluble partner, typically a bacterial protein; 2) co-expression of folding catalysts and chaperones; 3) expression under culture conditions which reduce the translation rates or affect the intracellular environment; and 4) modification of the protein sequence.
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#180741 Protein expression issue
Posted by
on 11 September 2018 - 11:44 PM
in
Protein Expression and Purification
#180383 Percentage of Outer Membrane Protein
Posted by
on 04 June 2018 - 10:48 PM
in
Protein and Proteomics
Pichia cell protein expression purification
Tag purification: His tag, affinity purified;
Ion exchange, gel filtration
It’s recommended you download this GE protein purification manual(sample preparation, protein purification, electrophoresis, blotting, and cell culture.) (https://www.gelifesc...m/en/us/support)
#180376 GLUT1 and GLUT4 expression in serum
Posted by
on 01 June 2018 - 12:09 AM
in
Protein Expression and Purification
Do you want to get this protein?
