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#180546 Measure ROS with 2,7 dichlorofluorescein (not diacetate!!!), its pos

Posted by autophagator on 18 July 2018 - 03:59 PM in MTT, Proliferation and Cytotoxicity Assay

The oxidant-sensing fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) is a nonpolar dye, converted into the polar derivative DCFH by cellular esterases that are nonfluorescent but switched to highly fluorescent DCF when oxidized by intracellular ROS.

 

But, In the lab, only we have 2-7 dichlorofluorescein (DCF), not diacetate!!!!  does anyone know if it can be measure and how? is there any protocol to do it with DFC.  

 

Thank in advance.

 





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