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Lydiayi's Content

There have been 5 items by Lydiayi (Search limited from 23-September 18)


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#17051 Why are my E. coli cells not pellet-able?

Posted by Lydiayi on 26 February 2009 - 12:25 PM in Microbiology

Hey, HomeBrew,

That's very interesting! Can you give me the citation of your paper published on that?
Thank you for your input!

Lydia

We had a mutant that over-produced an exopolysaccharide. We could not, for the life of us, get this strain to pellet; this phenotype was actually how we found what proved to be a very interesting EPS locus and regulatory mechanism in our bug.




#16893 diffrence between CV and resume

Posted by Lydiayi on 25 February 2009 - 09:03 AM in Resume, Recommendation Letter and Interview

Haha, I guess the "real world work experience" only means the industrial experience.

Nabi,

If I got it right, the "real world work expeience" means all the academic exprience, such as education, teaching and research conducted dont count. Only those industry experience do?

might be. I have no idea but thought it was amusing. Going back to the original query - I also wonder what is the difference between CV and Resume.




#16889 Why are my E. coli cells not pellet-able?

Posted by Lydiayi on 25 February 2009 - 08:46 AM in Microbiology

Thanks. Lower density or higher density would make it non-sticky?

I grew the cells at 37'C overnight and obtained a reasonably high amount of cells from 500 mL LB/amp, compared to normal fermentation of cells expressing the wild-type protein.

change in cell density. Change in the number of cells in solution, are also possible alternatives.




#16887 diffrence between CV and resume

Posted by Lydiayi on 25 February 2009 - 08:41 AM in Resume, Recommendation Letter and Interview

Hey, there won't be much difference between CV and resume if you're a Ph.D.

Resume is more like a description of your experience and highlight of your skills and specialties. It's often used in industrial job application. It could be very detailed about the projects you have worked on, but usually limit in length - 1~2 pages.

CV is a chronicle arrangement of your professional experience. It could be as long as you want. Usually it is used in academic settings. But scientists in industries also use CV.



#16883 Why are my E. coli cells not pellet-able?

Posted by Lydiayi on 25 February 2009 - 08:11 AM in Microbiology

Hey,

I'm expressing a lipopolysaccharide biosynthetic enzyme in E. coli. This is a enzyme from C. burnetii chimerized with a short C-terminal sequence from its E. coli homolog. The recombinant protein expressed as cytosolic oluble protein in E. coli BL21(DE3). But interestingly, comparing to the wild-type C. burbetii enzyme, this protein seems to make the E. coli cells unable to pellet well.

Wild-type C. burnetii enzyme/BL21(DE3):
-- Spin down at 6500 g for 10 min after fermentation overnight.
-- Formed nice pellet in the centrifuge bottle.

C. burnetii enzyme hybridized with a E. coli tail/BL21(DE3):
-- Spin down at 6500 g for 10 min after fermentation overnight.
-- Cell looks unsticky to each other and can't pellet down.

This happened several times. What would make the E. coli cells non-sticky? A change in morphology? Changes on the outer membrane? Anyone has any thought?

Thanks,

Lydia




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