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quanzeng's Content

There have been 5 items by quanzeng (Search limited from 20-July 18)


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#11280 Help: About the synthesis of protein bead.

Posted by quanzeng on 22 February 2005 - 10:27 AM in Protein and Proteomics

Hi everyone,

I am trying to prepare some protein particles for tissue engineering. Basicly, I want to synthesize collagen particles with diameter of about several microns. Anyone knows how to do it?

I appreciate your sugestion!!



#9827 Anyone can recomand me at least how many cells do I need?

Posted by quanzeng on 07 January 2005 - 08:42 PM in Protein and Proteomics

I am going to do some proteomics research of human cell. I am going to use 2-D gel electrophoresis followed by MS. Since I do not have much cells, I want to know a roughly number fefore I begin. Anyone can recomand me at least how many cells do I need?
Thank you!



#9824 How many cells I need for proteomics of human cells

Posted by quanzeng on 07 January 2005 - 02:29 PM in Cell Biology

I am going to do some proteomics research of human cell. I am going to use 2-D gel electrophoresis followed by MS. Since I do not have too much cells, I want to know a roughly number. Anyone can recomand me at least how many cells do I need?
Thank you!



#9823 The function of EDTA for harvest cells

Posted by quanzeng on 07 January 2005 - 01:30 PM in Cell Biology

Thank you for your reply!



#9800 The function of EDTA for harvest cells

Posted by quanzeng on 06 January 2005 - 08:00 PM in Cell Biology

Dear all,
I am going to analyze the proteins of whole cell. The cells are cultured on surface of T-flask. usually, I use Trypsin to harvest cells. However, it is said ENTA is better since it did not destroy the surface proteins.
Anyone can tell me how does EDTA work? I want to know the mechanism.
Thank you!!




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