Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

guayapa's Content

There have been 1 items by guayapa (Search limited from 22-September 19)

Sort by                Order  

#177085 Ligation: insert & vector are about the same size

Posted by guayapa on 03 November 2015 - 10:14 AM in Microbiology

Hello everybody,


I ligated an insert (3kb) with two different vectors that are both about 3kb. For one of the vectors the ligation worked out, for the other I got only empty vectors. Might this be due to the fact that vector and insert are about the same size? Do you have any other ideas? I tried general troubleshooting as proposed in manufaturer's instrutions.

Thanks in advance,


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.