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guayapa's Content

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#177085 Ligation: insert & vector are about the same size

Posted by guayapa on 03 November 2015 - 10:14 AM in Microbiology

Hello everybody,

 

I ligated an insert (3kb) with two different vectors that are both about 3kb. For one of the vectors the ligation worked out, for the other I got only empty vectors. Might this be due to the fact that vector and insert are about the same size? Do you have any other ideas? I tried general troubleshooting as proposed in manufaturer's instrutions.

Thanks in advance,

guayapa





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