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#175354 Loss fluorescence when clone a fluorescent sensor into a differenet vector

Posted by yusheng0104 on 10 June 2015 - 11:12 AM in Molecular Cloning

Dear all


I tried to clone a fluorescent sensor into another vector. I added a linker SAGGSAGG and a flag tag like sequence between the targeting sequence and the sensor. But the new construct just loses fluorescence. What could be the reason behind?


Thanks a lot

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