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GalkaPiteskaya's Content

There have been 2 items by GalkaPiteskaya (Search limited from 02-April 19)


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#169750 Best technique to analyse methylation level of CpG island

Posted by GalkaPiteskaya on 31 July 2014 - 01:00 AM in DNA Methylation and Epigenetics

Thank you! You never had problems even if it was CpG rich regions?




#169722 Best technique to analyse methylation level of CpG island

Posted by GalkaPiteskaya on 30 July 2014 - 06:01 AM in DNA Methylation and Epigenetics

I have used bisulfite sequensing to analyse methylation level of several CpG sites in a region of interest. Length of most of my amplicons did not exceed 400bp and they were not CpG site dense. But now I would like to analyse the methylation level of several CpG islands with length 800-2000 bp and very CpG sites dense. As I know CpG dense regions are quite difficult to amplify. So I do not know if it makes sence to try another technique which is based on bis-DNA amplification, melting curve assay for example. Which another approach might be used here?





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