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#160354 Adshuttle vectors and adenoviruses

Posted by Vector1211 on 18 September 2013 - 07:42 AM in Molecular Biology

Does anyone have any experience working with Adshuttle vectors? Can someone please explain how an Adshuttle vector works in terms of producing adenovirus?

 

Here is what I need to do: Clone X sequence from a retroviral vector into an Adshuttle vector with a MCS and a Rat insulin promoter. I essentially need to create adenovirus to be able to do this but I'm not very familiar with the process and what I need to look for before I begin. Do I simply start with amplifying my sequence from the retroviral vector, following by restriction enzyme digest and the cloning process?

 

Any help is much appreciated! 





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