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#7607 Protein-protein-interaction

Posted by utopia on 02 October 2004 - 01:19 PM in Protein and Proteomics

hey folks,
can anybody help me please, which one of these methods is the most efficient one to showing an ínteraction bwn 2 proteins?

*Yeast 2-hybrid-system
*affinity chromatography
*co-immunopricipation
* ??or do you have a better idea?

Immunohistochemically, we could show the colocalization between 2 membranproteins. The question is now: do these proteins interact with each other directly and therefore we get a colocalisationsignal or they are just in close neighbourhood of each other and due to this adjacency we get a signal. :)

which method have I to use in order to be able to prove the colocalisation?

thank you in advance
Shokufeh




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