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BMF's Content

There have been 14 items by BMF (Search limited from 16-April 20)


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#177981 Health Canada asked us to use anti-UV glasses for UVB and not UVC

Posted by BMF on 06 April 2016 - 08:25 AM in Venting and Counseling

Health Canada asked us to use anti-UV glasses for UVB.

 

In our experiment we use UVB lamps under biological hoods and our distance is more than 2 meters from lamps and  UVB/A meter shows that the amount of UVB/A combine is less than 100 micro Wat per square cm from 2 meters and is 50 micro Wat per square cm from 3 meters.

 

Another funny part of this story is that all flurescent lamps on the ceiling has 25 micro Wat per square cm.

While outdoor total UVB/A combine in sunlight is 8900 in Feb and 15000 in June.

When you sit near a window in sunny days, you receive 400 indirectly (in shadow) and 3000 directly (in sunlight passed from glass of  windows).

 

But the stupidity of this rule is that in all cell culture rooms and biological hoods there are UVC lamps which is the the most dangerous UV and there is no rule for using glasses for going to cell culture rooms in Canada.

and all cell culture rooms and biological hoods have no cover to block UVC waves.

 

I wonder who has selected these stupids as authorities in  Health Canada.

Whenever they start taking, you can quickly understand that they do not know what is UV  and its kinds.

 

As I mentioned before in link below, Canada is the land of stupidity:

http://www.protocol-...s-not-care-abo/




#177969 Program to draw plasmids

Posted by BMF on 03 April 2016 - 06:12 PM in Bioinformatics and Biostatistics

https://babakmemari....lysis-software/




#177949 What should I do in front of a McGill University Professor who does not care abo

Posted by BMF on 30 March 2016 - 07:13 AM in Venting and Counseling

In Canada, educated people have low knowledge, especially authorities.

And the problem is they do not care too.

 

This is why the Canadian are successful in two jobs in US, because these jobs need low knowledge and talkative persons:

1) Comedian 2) Singer

 

Those who think Canada is the best and the Canada Health checks every thing, should see this:

 

Toxic jewelry: What’s in the cheap jewelry you buy (some had 100% cadmium even in Sears)

http://www.cbc.ca/ma...6/toxic-jewelry

 

 

Canada does not do anything. They just sit and wait for US authority's decisions.

These two happened during past 3 years:

If US warns for a contaminated beef, 3 days after that, Canada warns for it.

If US warns for Tsunami, 3 days after, Canada warns for it.




#177781 Reference gene(s) selection and validaton for qPCR

Posted by BMF on 01 March 2016 - 12:59 PM in PCR, RT-PCR and Real-Time PCR

Sean Taylor’s Guideline for qPCR

 

https://www.karger.c...FullText/356189

 

https://babakmemari....delinefor-qpcr/




#177642 What should I do in front of a McGill University Professor who does not care abo

Posted by BMF on 11 February 2016 - 05:34 PM in Venting and Counseling

Another funny papers that all primers are wrong except one:

 

Impact factor 6 and Cited by 188 since 2009 until now:

 

http://atvb.ahajourn...t/29/6/936.full

 

this paper should have been in retractionwatch.com




#177518 total number of restriction enzyme recognition sequence in human genome ?

Posted by BMF on 17 January 2016 - 05:07 PM in Bioinformatics and Biostatistics

how to find the total number of recognition sequence of a restriction enzyme in total human genome ?




#177274 Lower Plasmid Yields

Posted by BMF on 26 November 2015 - 02:10 PM in Molecular Cloning

Can you try this and inform us:

 

culture it on plate for less than 18 hr to have single colonies by loop.

Then culture positive ones and check amount of plasmids.




#176977 Are Bio-rad gene-pulser cuvettes compatible with a Lonza nucleofector 2b system?

Posted by BMF on 21 October 2015 - 07:09 AM in Tissue and Cell Culture

Base on this:

http://www.ispybio.c.../bacteria_6.pdf

 

You can use:

Standard 1 mm electroporation cuvettes (e.g. from BioRad, Eurogentec, Eppendorf, Cole-Parmer, BTX,
Epicentre, Genlantis, Sigma, Thermo Electron)




#176649 RNA extraction for RNAseq

Posted by BMF on 08 September 2015 - 10:40 AM in Molecular Biology

My favorite way is this:

 

Liquid Nitrogen Cooled  Mortar & Pestle

http://www.braintree...?number=3726001

 

Some image for how to do it:

 

https://www.google.c...wIVBF2SCh0VjwJu

 

Then mix the powder of tissue with Trizol or any solution from any KIT for RNA extraction.




#176438 Good parafilm

Posted by BMF on 18 August 2015 - 12:13 PM in Microbiology

For this Cat Number 01816E, you can put 6 petri dishes (10 cm).




#176427 Good parafilm

Posted by BMF on 18 August 2015 - 07:31 AM in Microbiology

This Zip locker bag are very thick and it has been used for  Mycobacterium tuberculosis (Obligate aerobe       https://en.wikipedia...Obligate_aerobe) culture on plates for 14 to 30 days with no problem.

 

01816E

https://www.fishersc...ctDetails_32048




#174695 What should I do in front of a McGill University Professor who does not care abo

Posted by BMF on 04 May 2015 - 09:32 AM in Venting and Counseling

Ok this is a sample from Nature:

 

http://www.nature.co...ary-information

Primers are in this link:

Supplementary Table 10. Primer sequences

http://www.nature.co...ure13684-s2.zip

 

Just check the B2m for mice and CXCL2.

B2m does not bind at all.

 

https://i.imgur.com/bDsRUJu.jpg

https://i.imgur.com/t9qGkHl.jpg

https://i.imgur.com/Mu3qRtc.jpg

 

 

Babak




#174625 What should I do in front of a McGill University Professor who does not care abo

Posted by BMF on 29 April 2015 - 11:09 AM in Venting and Counseling

when you do Primer-Blast for a primer from a 13 impact factor journal in 2014.

And these primers bind everything except the gene of interest, you say again this:

 

"sometimes it happens. It doesn't have to be necessarily fake data.."




#174619 What should I do in front of a McGill University Professor who does not care abo

Posted by BMF on 29 April 2015 - 07:58 AM in Venting and Counseling

I have a paper reading course in McGill University for PhD students.

A Professor is in charge of this course. I usualy find a msitake in primers in paper in average one out of 15 papers, so that these primers do not bind to that gene of interest in paper and so that data is fake.

 

But this professor laughed at me and siad in front of students:  Haha ha  he (me) likes primers.

Actualy this is not the first one. Also I found that he does not know what primer is and what it is for. and how to check primer.

 

What should I do? After 30 years from invention of primer?





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