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volume of a 96well is 150ĩl
the difference is the diameter of the well. in a 24well diameter is about 4fold greater.
So it is 150ul and not 150ml? If the well of a 24 well plate has greater diameter then why it has less volume ?
How can i feed suspension cells?
Are the cells from the hypophysis tissue cells or neural cells? And do the general cell culture rules also apply to the neural cells?
poly a sequence upstream a reporter gene is for ensuring the good RNA termination, and translation.
Usually the poly A sequence lays downstream of a gene to give it good RNA termination and translation. BUt when it lies upstream is something new . Upstream means before the promoter. How come?
Are all cancer cells immortal cells? Or are there only cells that express an immortal protein that become immortal cells whether they are normal cells or cancer cells?
be careful. there is no law to all this (cell lines themselves are anarchists)...
of course immortal cell lines can change their character. they donīt do it that easily. it depends... on the growth conditions/handling, the cell line etc.
sorry itīs not that easy.
This is the first time i will work with this technique, but i can't find any info or good links about it. I would like to know about it as much as i can. I would love to have them all. This would help me alot in my experiments. Thank you very much.
Lemme know what kind of info you need, Protocols? Just basic Info? Advanced Data Analysis stuff? I have viewed and used info from a lot and I might be able to give you some links.
so after my frozen stock has reached the last passage number then i should get a new vial from the supplier? what if i freeze down the last passage number and keep thaw and passage it? will my results get affected?
well, officially you got 6 passages left...counting down...3...2...1 - pay another 400 bucks for a fresh vial from your favourite supplier...
what do you mean?
ever since i started working with cell lines, i have been wondering if people do it that way in real world.
thanks for any inputs.
1. spin down the remaining cells.
2. resuspend cells with 1.5ml medium.
3. add 1.5ml 2X freezing medium.
4. freeze down cells in 1.6ml cryovials.
my question; why should i add 1.5ml medium (step 2) when my vial only has a total volume of 1.6ml. should i ignore step 2? any ideas?