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Zac70's Content

There have been 2 items by Zac70 (Search limited from 17-February 18)


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#148804 Human monocyte derived macrophages from PBMC's

Posted by Zac70 on 25 January 2013 - 11:47 AM in Tissue and Cell Culture

Could some who has done this procedure before share with me their protocol in detail please including Ficoll gradient, adherence conditions and culture conditions (m-csf amount etc) for differentiation?

Thanks for any help or comments.



#148803 need a comprehensive protocol for isolating and culturing human monocytes

Posted by Zac70 on 25 January 2013 - 11:43 AM in Tissue and Cell Culture

Could some who has done this procedure before share with me there protocol in detail please.




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