Hey guys, so I'm having some trouble editing micrographs for my figures in an upcoming paper.
We have cells with different regions fluorescing different colors, and my collaborator sent me the greyscale images of these cells. I know how to simply merge pseudocolor images by RGB channel, but the problem is the images I was sent aren't perfectly aligned. What I need is the ability to slide the two images over one another so that they are properly aligned. I have done this in the past, but I believe it was with native microscope software I no longer have access to. Do y'all know of a way for me to do this with ImageJ, Inkscape, or Gimp? Or do I need to have my collaborator mess with it with his software?
It's easy for me to adjust the opacity of one image and slide it over the other, but then I have to worry about how the background obscures the image I am merging into. Does this make sense? Thanks for any help.
No need to double post - I've deleted your other one. Bob