In this instance I can't count the cells as the they have to be confluent and are too packed to count individual cells. I was thinking it may be possible to take a picture of the whole well in both GFP, then CHERRY and use some software to determine the percentage of each. I will just have to scale it down to 96 well plates (not 12 well). Do you think this is possible?
I am co-culturing cells labelled with GFP and cells labelled with CHERRY. I want to determine the percentage of each colour before and after treatment with a compound. FACS is not an option. Does anyone know how this can be done?