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c0zmic's Content

There have been 2 items by c0zmic (Search limited from 25-October 19)


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#137876 Determining the % of GFP/CHERRY cells in co-mixed culture

Posted by c0zmic on 18 July 2012 - 11:44 PM in Cell Biology

In this instance I can't count the cells as the they have to be confluent and are too packed to count individual cells. I was thinking it may be possible to take a picture of the whole well in both GFP, then CHERRY and use some software to determine the percentage of each. I will just have to scale it down to 96 well plates (not 12 well). Do you think this is possible?



#137804 Determining the % of GFP/CHERRY cells in co-mixed culture

Posted by c0zmic on 18 July 2012 - 03:44 AM in Cell Biology

I am co-culturing cells labelled with GFP and cells labelled with CHERRY. I want to determine the percentage of each colour before and after treatment with a compound. FACS is not an option. Does anyone know how this can be done?

Any advice would greatly be appreciated.




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