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yoohoo's Content

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#130308 Lipofectamine transfections once working now dying

Posted by yoohoo on 02 March 2012 - 11:46 AM in Cell Biology

I have been using Lipofectamine 2000 and Lipofectamine LTX myself. How long do you let your Lipofectamine stay out at room temperature? The liposomes may degrade over time There is a simple method of fixing this by using an ice block. Ensure that the tube doesn't remain out for too long.

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