Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

C.T's Content

There have been 1 items by C.T (Search limited from 15-April 20)

Sort by                Order  

#129514 Culturing Human CD14+ monocytes

Posted by C.T on 19 February 2012 - 10:24 AM in Tissue and Cell Culture

I have been having some problems with my monocyte cell cultures and wondered if anyone had any ideas.

I separate PBMCs from human blood using histopaque, then use miltenyi CD14 beads to get the CD14+ cells. After 48hr cell culture between 40-50% of my cells are dead.

I culture in RPMI supplemented with 10% FCS, L-glut and pen/strep, and have also tried adding 2-ME - with no benefit.

Any ideas to increase monoccyte cell survival?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.