100 ul of suspension that has 10_6 cells per 1000 ul contains 10_5 cells
1ug of antibody per 1000 ul so that means 0.1 ug for the 100 ul cells you have
your stock is 200 ug antibody per 1000 ul, and you just saw that you need 0.1 ug for the experiment...can you figure out the rest ?
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#173309 Antibody concentration
Posted by
on 10 February 2015 - 10:11 PM
in
Flow Cytometry
#173305 Antibody concentration
Posted by
on 10 February 2015 - 04:05 PM
in
Flow Cytometry
can you show me how to calculate?
#173295 Antibody concentration
Posted by
on 10 February 2015 - 05:38 AM
in
Flow Cytometry
sorry, i m not understand. can u make it clear ?
#173292 Antibody concentration
Posted by
on 09 February 2015 - 10:53 PM
in
Flow Cytometry
hello everyone
Need help .
Cell suspension that I have =100ul of cell suspension with concentration 1x10^6 cells/ml
Concentration Antibody that I have (stock@purchased)= 200ug/ml
Concentration Antibody that have to be used in experiment= 1ug/1x10^6 cells
from supplier datasheet (Antibody): dilution= 1ug/1x10^6 cells.
How much volume I need to take out from the stock antibody to be used in my experiment?
#171591 Chamber Slide
Posted by
on 07 November 2014 - 10:34 PM
in
Tissue and Cell Culture
Thank you.
I already used it. now the treatment is going on.
it is not wrong if I use either one, plate @ chamber slide for staining (cell lines)purposes right?
#171513 Chamber Slide
Posted by
on 02 November 2014 - 12:36 AM
in
Tissue and Cell Culture
hello.
I am going to use Lab-Tek Chamber Slide 4 wells to grow cell line for staining purposes.
Does anybody has a video or link on how to use that slides, concerning how to discard media etc.
Thank you
