I'm trying to characterize a (very) low rate nuclease using hyperchromicity and it gives me headaches . The definition of a (kunitz) Unit using this methodoloy is not very clear, as depending on the source, people are using different substrate concentrations and assay conditions. I generally do my assays using 50µg/ml gDNA in the optimal pH and cofactor conditions for my enzyme. My question is whether I should calculate the activity by multiplying the mAU260nm/minxmg by 20 to extrapolate to 1 mg/ml gDNA substrate?
Thank you so much for your suggestions