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valen's Content

There have been 5 items by valen (Search limited from 21-January 19)


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#113346 Erythromycin LB agar

Posted by valen on 23 June 2011 - 05:09 AM in Microbiology

Hi,
I am using pTRKH3 plasmid http://bccm.belspo.b...s.php?NM=pTRKH3 .
I am trying to clon a cDNA (fused to GFP) in pTRKH3 but when I do the transformation (using DH5alpha) with Erythromycin (150ug/ml or 250ug/ml) the plate is full of bacteria (there are no colonies).

Could you tell me what's going on?

Many thanks in advance,
Valen



#113345 Erythromycin LB agar

Posted by valen on 23 June 2011 - 05:07 AM in General Lab Techniques

I am using pTRKH3 plasmid http://bccm.belspo.b...s.php?NM=pTRKH3 .
I am trying to clon a cDNA (fused to GFP) in pTRKH3 but when I do the transformation (using DH5alpha) with Erythromycin (150ug/ml or 250ug/ml) the plate is full of bacteria (there are no colonies).

Could you tell me what's going on?

Many thanks in advance,
Valen



#111483 monolayer lactobacillus

Posted by valen on 01 June 2011 - 04:11 AM in Microbiology

Hi

I would like to ask whether any of you tried to culture any bacteria in tissue culture flask? does the lactobacillus stick to the flask?

thanks,
Valen



#109371 Lactobacillus plasmid

Posted by valen on 10 May 2011 - 07:51 AM in Molecular Biology

Hi,

I want to express a GFP-fused protein in lactobacillus. What plasmid would you suugest me to use?

thank you,
Valen



#109230 pGFP-C2 & Lactobacillus

Posted by valen on 09 May 2011 - 05:08 AM in Molecular Biology

Hi,

Please, could anyone tell me whether pGFP-C2 (Host: E. Coli) could work for Lactobacillus casei?

Many thanks for your comments.
Valen




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