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zhongw90's Content

There have been 4 items by zhongw90 (Search limited from 19-February 19)


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#8768 help - stable transfection clone pick up

Posted by zhongw90 on 19 November 2004 - 09:21 AM in Cell Biology

how to remove the trypsin after detaching the cells? I doubt if centrifugation is appropriate since there are so few cells.


You don't need to remove the trace trypsin.



#5306 help - stable transfection clone pick up

Posted by zhongw90 on 08 April 2004 - 09:23 AM in Cell Biology

Hello Geness,

There are many ways of picking clones

You can use a cloning cylinder (such as from Fisher)
To isolate incubated colonies, insert cloning cylinder around the cell colony and attach by sterile grease. Add trypsin to the cylinder and incubate until cells detach from the dish. Collect cells by a Pasteur pipet and transfer to separate vessel.

Or simply, without using cylinder, you can add trypsin to a well isolated cell clone and collect cells by pipetting.

Also have a look at this link http://anatomy.med.u...ethods/mh10.htm

Good luck.

ZW



#5058 why are most species symmetrical externally

Posted by zhongw90 on 18 February 2004 - 11:01 PM in Chit Chat

by chance or by design?

about origin of species, evolution or intelligent design, which do you believe?



#4934 microarray replica

Posted by zhongw90 on 22 January 2004 - 09:58 AM in Molecular Biology

Hi all,

I want to study gene expression changed by treatment in a cell line. I will study 3 time points. My question is how many replica are necessary, at what stage, such as at treatment, RNA extraction, hybridization.

thank you.

ZW




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