Sorry, I had a slow moment... Won't adding b-mercaptoethanol denature my proteins, making it pointless for a NativePAGE gel?
I'm trying to determine the size of my proteins in their native state. Since gel filtration didn't work, my next option was to try this Novex NativePAGE gel system (charges the proteins with coomassie). Because of the streaking, it hasn't been helpful either.
I have been running blue Native PAGE gels using 3-12% bis-tris gels. I keep getting protein streaking, and no matter what I do, it won't go away. I have reduced the salt concentration of my samples, used the 5% G-250 coomassie sample additive, and spun the samples the remove any particulates, and reduced the amount of protein in my but the streaking endures. Any thoughts as to what could be causing it?