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qPCRman's Content

There have been 7 items by qPCRman (Search limited from 17-July 18)


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#97286 qPCR Data analysis

Posted by qPCRman on 12 January 2011 - 07:25 AM in PCR, RT-PCR and Real-Time PCR

Hi-

GenEx is quite good- I used it. You may also want to check RealTime StatMiner- its quite user friendly where they follow the Livak paper.

Look up the whitepaper: http://www.integromi...qPCR_Statistics

Sam



#97284 qPCR data analysis

Posted by qPCRman on 12 January 2011 - 07:20 AM in PCR, RT-PCR and Real-Time PCR

Hi-

Look up the whitepaper: http://www.integromi...qPCR_Statistics and the Livak paper. They are very useful.

Sam



#97282 delta Ct values

Posted by qPCRman on 12 January 2011 - 07:16 AM in PCR, RT-PCR and Real-Time PCR

Hi-

Look up the whitepaper: http://www.integromi...qPCR_Statistics and also Livak paper.

Sam



#97281 Alternative software to read .sds files?

Posted by qPCRman on 12 January 2011 - 07:14 AM in PCR, RT-PCR and Real-Time PCR

Hey,

You can export the results or use amplification curve data and import to RealTime StatMiner.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

Sam



#97279 delta-delta-Ct method

Posted by qPCRman on 12 January 2011 - 07:09 AM in PCR, RT-PCR and Real-Time PCR

Hey

You can replace it with Ct=40 (max number of cycles). I use RealTime StatMiner software and it does it automatically. You can set a filter to see how many times this appeared Undetemined amongst your biological replicates. Undetermined may also suggest you don't have anything in your control, so that may be significant. You just have to setup your filters.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

and also the Livak paper.

Sam



#97277 How to present and analyze these real-time PCR data?

Posted by qPCRman on 12 January 2011 - 07:05 AM in PCR, RT-PCR and Real-Time PCR

Hey

You need to normalize the Cts to the endogenous control- get the DCt and then compare the control to the target group for fold change.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

and also the Livak paper.

Sam



#97275 Ct value and interpretation

Posted by qPCRman on 12 January 2011 - 06:59 AM in PCR, RT-PCR and Real-Time PCR

Hi-

You need to normalize the Cts to the endogenous control- get the DCt and then compare the control to the target group for fold change. You can calculate the DDCt with two samples.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

and also the Livak paper.

Sam




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