I am working with black 6 mouse splenoytes. I did total splenocytes migration and I am looking specifically for Th17 subset. I used 10%FBS for normal cell culture. I read it somewhere says migration assay should avoid FBS becuase the cell will migration nonspecifically that's why I used BSA but they died.
I am new in doing migration assay. I have problem maintain the cell viability after 3 hr of migration. I only get 8% to 12% cell that are alive after migration. I used 0.5 BSA migration medium and 3% FBS migration medium and FBS give me just a little better viability. I just don't understand how they all die after just 3hr of migration. Can someone give me some tips on migration assay please?
Hi I have a bottle of IL-2 cytokine from R&D system (402-ML) and it is 20ug. I need to calculate how much I need to get 100 U/mL. On R&D website, it says the ED50 is 0.5ng/mL and the its 2.5X10^5 U/ug. Can someone help me please!