Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

yi198720022004's Content

There have been 6 items by yi198720022004 (Search limited from 04-August 19)


Sort by                Order  

#129445 Problem with transwell migration

Posted by yi198720022004 on 17 February 2012 - 09:57 PM in Cell Biology

I am working with black 6 mouse splenoytes. I did total splenocytes migration and I am looking specifically for Th17 subset. I used 10%FBS for normal cell culture. I read it somewhere says migration assay should avoid FBS becuase the cell will migration nonspecifically that's why I used BSA but they died.



#128977 Problem with transwell migration

Posted by yi198720022004 on 12 February 2012 - 06:43 PM in Cell Biology

Hello,

I am new in doing migration assay. I have problem maintain the cell viability after 3 hr of migration. I only get 8% to 12% cell that are alive after migration. I used 0.5 BSA migration medium and 3% FBS migration medium and FBS give me just a little better viability. I just don't understand how they all die after just 3hr of migration. Can someone give me some tips on migration assay please?

Thanks in advance!



#121026 Convert unit to ul

Posted by yi198720022004 on 06 October 2011 - 03:46 PM in General Lab Techniques

Thank you for your help. I get it now:)



#120975 convert IL-2 unit to mass

Posted by yi198720022004 on 06 October 2011 - 05:43 AM in Immunology

Hi I have a bottle of IL-2 cytokine from R&D system (402-ML) and it is 20ug. I need to calculate how much I need to get 100 U/mL. On R&D website, it says the ED50 is 0.5ng/mL and the its 2.5X10^5 U/ug. Can someone help me please!



#120974 Convert unit to ul

Posted by yi198720022004 on 06 October 2011 - 05:40 AM in General Lab Techniques

On the R&D websites. It says the ED50 value is 0.5ng/mL. and its 2.5X10^5 U/ug. Thank you!



#120961 Convert unit to ul

Posted by yi198720022004 on 05 October 2011 - 07:45 PM in General Lab Techniques

Hi I have a bottle of IL-2 cytokine from R&D system (402-ML) and it is 20ug. I need to calculate how much I need to get 100 U/mL. Can someone help me please!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.