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a.harv's Content

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#89232 H9C2 maximum pasage

Posted by a.harv on 11 October 2010 - 07:04 AM in Tissue and Cell Culture

I'm just wondering how many passages I can get out of the H9C2 cell line without any phenotypic changes occuring? I've read some differing things ranging from 25 to 40 for maximum passages, I'm using these cells for hypertrophy experiments with a view to transfect in the future.
Any input would be greatly apprecitated.
Adam




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