In some published paper, some people use 0.1%ethanol as vehicle control,but my 0.1%ethanol group were all dead, but 0.1%DMSO group was OK, maybe different resource, our MCF-7 cells were just sensitive to ethanol, now, I dissolved all my reagents in DMSO, my experiment is going on well. Good luck to you.
I dont know what is Incap cells, do you mind explaining?
I have similar problem with MCF-7. I start to notice that MCF-7 is might be sensitive to ethanol. I made it custom to spray ethanol on the body of the flasks everytime removing or keeping it in the incubator. I try not to spray the caps. I notice that my media gets cloudy in 24h as the cells lysed. A PHD student recommend me not to spray anymore, when I stop spraying, the condition get so much better, MCF7 becomes easily confluent. Why?
I posted my question as new topic just now, maybe you could give some of your opinion.
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