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Iwenim's Content

There have been 2 items by Iwenim (Search limited from 29-May 19)


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#141214 Live/Dead Staining of already Permeable Cells

Posted by Iwenim on 12 September 2012 - 02:07 AM in Flow Cytometry

Hello Martin,
there is the possibility to stain your cells with EMA (Ethidiummonoazid) before.
EMA is binding covalent on the DNA.
Add EMA to the cells incubate about 10 in the dark on ice and further 20 min of incubation under strong light.
Then wash the cells and go on with your normal treatment.

Furthermore there are Live/dead cell staining kits from companies, using different dyes than EMA.

Hope that helps.
Iwenim



#139382 Gradient-gel for Western Blot

Posted by Iwenim on 13 August 2012 - 06:21 AM in SDS-PAGE and Western Blotting

Hello everybody,
I would like to pour gradient gels for western blot. I just got an Model 495 from BioRad for pouring these gels.
Does anybody has experience with this gradient mixer and knows how to use it? I have the instructions but it is not very helpful.

I would like to pour 14x20 cm gels.
Thanks in advance!
Iwenim




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