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There have been 27 items by PandaCreamPuff (Search limited from 22-July 18)
Writing is going alright....I have 2-3 publications lined up for my supervisors to read...but they decided that teaching is more important than research at the moment...so they have actually stopped reading emails and turned off their phones....it's SO FRUSTRATING
and never mind about my thesis drafts....it's less than 5 months away and they have only read like 5% of what i have typed...which is probably 80% of the whole thesis >_<
I am at a point where:
1) I have no hope whatsoever about my chances for my degree
2) Even if I failed I would not feel as bad as I know I have tried my best, and I failed based on things outside my control.....hopefully this is not the case in the end though.....
I am writing up my thesis and wrote "islet" on a section of the chromosome containing multiple genes (loci), but then my supervisor circled islet and said "you mean locus? how different?" Therefore, I'd like to make sure that and islet is not just one locus but a region of multiple loci....if that is the case, then what is the difference between an islet and an operon?
I have nucleotide sequences which I would like to BLAST, however I would not like to BLAST the whole database, instead just a selected series of sequences. I would like to know how to get around that. Can I limit my search to only include some accession numbers?
Thank you so much
I am aware of these translators, however I was more asking about whether there are services which are catered to the scientific community. I am not sure how reliable these translation products in terms of accurately conveying the messages in the publication.
I have this important paper that is published in German. Do you guys know of any translation services out there? I am highly reluctant on using google translation, however I have found this website, and am wondering if any of you has used it before:
I am making a 2 x 4 contingency table, but one of the cells has an expected frequency of less than 5. Therefore I cannot use the chi-square to compare observed vs expected ratio, and will need the use the Fisher's Exact Test.
Most of the Fisher Test tutorials talk about 2 x 2 contingency tables, but I am not sure how to fit this into a 2 x 2 table...
Outcome 1 Expected: 6
Outcome 2 Expected: 4
Outcome 1 Observed: 0
Outcome 2 Observed: 10
Can someone let me know how to get around this?
Thanks so much all
I am trying to determine if there is an association between the extent of sequence divergence of gene "A" between two species and the extent of divergence of gene "B." I would have one axis with the number of nucleotide mismatch of gene A, and another axis of gene B.
Is there a software that allows me to put in my sequence data, perform pairwise sequence analysis of both eachs in each paired combinations, and plot that as a regression analysis?
Thank you so much
I would like to generate a tree based on multiple DNA sequence alignment from ClustalW. I have not trimmed my sequences, but under the default ClustalW setting, does it only calculate the similarities on the region where all sequences are aligned? In another words, if I have some sequences that are longer than others, will these regions be calculated as gaps?
I have posted an image of my alignment for visual aid: will the gap seen in sequence 2/1-829 be seen as differing from the other 3 sequences?
Thanks for any input.
I have been storing genomic DNA after a heat-lysis method in PBS. I have been doing this for some time and my PCR and sequencing seems to work fine. I am just worried that for my thesis this will cause me some mark deduction, as I did not use TE buffer, which is probably the conventional way of storing DNA. Has anyone stored gDNA in PBS?
I am currently getting my head around some concepts regarding horizontal gene transfer. The following paper looked at recombinational events and have identified an "x" number of gene transfers:
They then address that a big proportion of the recombination breakpoints corresponds to "gene borders," suggesting that both inter and intra species recombination take place.
My question is that what does a "gene border" mean in this sense? Does it relate to sequences bordering homologies to two different species in nucleotide sequences?
Thank you so much
Anyways, I am researching on a PCR-based molecular method for diagnosing etiological bacterial pathogens for mucosal illnesses. One aspect of PCR-based methods is that they do not require viable bacteria, as naked DNA can also be amplified if it's the targeted nucleotide fragment.
So my question is if anyone knows the duration of naked DNA persistence in the mucosal surfaces, specifically the respiratory tract? Because if it persists for a long time, the source of the naked DNA may not be present as an intact cell responsible for illnesses.
Thanks so much.
I just don't know what to think. Seems like no matter how hard I work I am not really improving. It's so tempting to just tell everyone I am quitting and forget about this whole annoying degree >_<
I know this may sound absurd and random, but I would like to know what I can eat during lunch times so that I can perform better in consolidating memories when I read scientific papers. Specifially, I think I need food that enhances my concentration. I would think avoiding carbs would help me get less sleepy....
I might just get fruits and vegs for lunch today.
PS: *runs to supermarket to get the freshest fruits now* >_<
So I am based in a UK lab trying to submit a paper to an American journal. My boss, by default, encourages me to write in UK English, however would it matter if I am submitting to an American journal? Would they want me to write in American English?
All these "organize" vs "organise" and "colonize" vs. "colonize" is doing my head in lol
Thanks a munch!
Looks good but do you have the article in pdf form? Biotechnique website has issues from 2004 in electronic format. I would like to check how to use this one under Mac.
Hi noelmathur try this?
I was under the impression that the convention is that bacterial strains can be obtained from ATCC or NCTC, however my boss insisted that I look at CDC. I did not dare asking him why not ATCC/NCTC. I looked at the CDC website and it's more like "oh you should wash your hand before dinner" that sort of website (more for the lay-people).
Does CDC actually have a reserve for bacterial strains where researchers can order? I learned from high school that they have the pox virus on reserve but other than that..... >_<
Thanks so much
I have two sequences of about 700bp, and I would like to see if a particular RFLP enzyme can differentiate them from each other on a gel. Is there a programme that I can use to tell me which enzyme I should use? I am currently tackling NEBcutter but not having much luck.