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There have been 4 items by Oxford (Search limited from 25-September 19)


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#57375 Mouse T cell line

Posted by Oxford on 02 February 2010 - 01:28 AM in Cell Biology

Thank you!

T cell hybridomas:
12H5, 3DO54.8, 3DO11.10, 8DO51.15, and 3DO18.3 (OVA specific)
3A9 murine CD4+ T-cell hybridoma (HEL specific)

Best,




#57275 Mouse T cell line

Posted by Oxford on 01 February 2010 - 01:25 AM in Cell Biology

Does anyone know of any mouse T cell lines?

Thanks



#54120 SNPs in introns

Posted by Oxford on 06 January 2010 - 08:39 AM in General Biology Discussion

Just wondering who the key people in the field of working-up SNPs in introns are?

Thanks for your time,

Adam



#54117 Transfection - Plasmid Uptake Control

Posted by Oxford on 06 January 2010 - 08:26 AM in Molecular Biology

Hi,

I'm transfecting HEK 293T cells by nucleofection and I want a control for the amount of plasmid taken up in each transfection.

I've read that this can be done by Southern blot but isn't very quantitative and is an out-dated technique.

The alternatives I've come across are cotransfections with reporter plasmids, but surely plasmid-reporter plasmid interactions and different capactity for uptake between reporter and construct could skew results?

I was wondering what is generally done in the field?

Thanks for your time,

Adam




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