I use real time PCR for the quantification of invironmental samples.
These samples contain many inhibitors and I wish to add a control test that varify the presence of inhibitors left
after the DNA purification.
I heard there is an "alien" probe sequence that is being used which suppose not to accure in any creature genomic DNA
known in the NCBI database.
I undersatnd that poeple use this sequence instead of there own probe sequence between the same specific primers they designed.
If anybody know how to get to these sequences or have any other good test for the persence of inhibitor I would love to hear about it.
I'm looking for specific amplification set for real-time PCR using TaqMan probe
that will differentiate between E. coli to other Enterobacteria (and other bacteria of course)
so far I found a 24bp sequence that is specific according to NCBI but can't find right primers to match for it.
I'll be happy to get any suggestions or refrences.
Thanks in advance