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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 17-January 19)



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#17063 Seeding volume in cell culture flasks

Posted by Minnie Mouse on 26 February 2009 - 01:41 PM in Cell Biology

In my hands, no.

The reason is when you lay the flask on its side during incubation, the amount of media flows too closely to the neck which could lead to contamination.

For suspension cultures, the largest volume I've used is 16 mL.
If you need larger volume, try a spinner flask or roller bottle.



yes your are correct i used only 15ml but i saw that we can make upto 40ml
i want to try...as it is not possible to keep the flask flat ...could it be possible to stand the flask ?
do the cells still be happy in that conditions with less length?


I don't think your cell will be happy.
If you stand the flask, the cell will accumulate at the bottom of the flask and reduce the area for them to grow.
The cells at the very bottom will not get nutrient from the medium. Unless you gently shake the flask every hour.

This is why people invented rolling bottle.

Hope this may help.



#31646 Scientific words

Posted by Minnie Mouse on 04 August 2009 - 03:49 AM in Scientific English

Would someone tell me which words are correct with hyphen, please? :(

overexpression or over-expression
upregulation or up-regulation
antitumor or anti-tumor
costimulatory or co-stimulatory
immunohistochemistry or immuno-histochemistry
downregulation or down-regulation
proapoptotic or pro-apoptotic
antiapoptotic or anti-apoptotic
prosurvival or pro-survival
chemoresistance or chemo-resistance
protooncogene or proto-oncogene


Thanks in advance.



#14871 Reuse DNA spin column

Posted by Minnie Mouse on 07 February 2009 - 02:55 PM in Molecular Biology

Thank you for the link, phage434.

Welcome back phage434.



#14341 Reuse DNA spin column

Posted by Minnie Mouse on 03 February 2009 - 02:08 PM in Molecular Biology

Reuse DNA spin column

Attached File  reuse_DNA_spin_column.pdf   440.14KB   3492 downloads



#14637 Reuse DNA spin column

Posted by Minnie Mouse on 05 February 2009 - 01:19 PM in Molecular Biology

Reuse DNA spin column

Attached File  reuse_DNA_spin_column.pdf   440.14KB   3492 downloads



Thanks Minnie mouse to post my paper (i am one of the author of this paper)


Thank you for publishing this paper. You have saved our money ;)

If you have further tips on reusing the DNA spin column, please post them here? :(

We would love to know :)



#19735 Reuse DNA spin column

Posted by Minnie Mouse on 23 March 2009 - 01:56 PM in Molecular Biology

thank you, labtek



#21704 Reuse DNA spin column

Posted by Minnie Mouse on 13 April 2009 - 02:55 PM in Molecular Biology

I am confused about the method exactly.

It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.

When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?

Also same thing about equilibrate. What does that mean exactly.


fill the column and centrifuge to get rid of the water



#27241 Research Assistant positions for semi fresh graduates like me

Posted by Minnie Mouse on 21 June 2009 - 08:12 PM in Venting and Counseling

Have you think about working overseas?



#27239 Re-using Culture flasks

Posted by Minnie Mouse on 21 June 2009 - 08:00 PM in Tissue and Cell Culture

are flasks THAT expensive you would mess up your results - just to recycle them? (taking into account the price of everthing else used leading to that point)

d



Dom,

Wonderful to have you back and talking common sense....your not my long lost brother?

Uncle Rhombus


Well, that all depends on where your funding comes from. I have to beg, borrow and steal just about everything because of a few expensive purchases in the beginning.
But yea, sure they are not THAT expensive to risk messing my results but I didnt know that until now.


I got the same problem during my PhD because I didn't have a sponsor to pay for my expensive PhD training cost :o .
I had to re-use the flasks for 5-10 times...not a good idea...but I did not have enough money to do experiments.

I know Uncle Rhombus will laugh at me. :(



#17473 rating

Posted by Minnie Mouse on 02 March 2009 - 06:48 PM in Chit Chat

lol......the same topic that you replied earlier had rating....the Taqman primer and probe.


I would like to know how many posts I'd have to get PM enabled. I was in the middle of giving advice to someone and waiting for the answer, oops.
And I am not such a fervent poster to quickly make the necessary amount.

EDIT: oooh it works.


I think PM is enabled when you reach "two blue squares".



#17589 rating

Posted by Minnie Mouse on 03 March 2009 - 01:25 PM in Chit Chat

I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)


It is back now.


Thank you, Bioforum



#17474 rating

Posted by Minnie Mouse on 02 March 2009 - 06:55 PM in Chit Chat

This feature was in the old forum, I'm quite sure of that. The geek forum has it (you can rate all the topics over there) and it's new. But for the tech forums, some subforums have and some don't, so it's really intriguing. And at least now we know that moderators are not culpable, there go our escape goats :D ....


I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)



#13517 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 27 January 2009 - 01:37 PM in Flow Cytometry

Practical Flow Cytometry, Fourth Edition


http://www.invitroge...-Cytometry.html



#19749 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 03:57 PM in Flow Cytometry

This e-book is >74MB. It took me a long time to download.



#19734 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 01:54 PM in Flow Cytometry

Thank you, yobou



#27240 PhD problems

Posted by Minnie Mouse on 21 June 2009 - 08:10 PM in Life as a PhD Student and Postdoc

Can you manage to use your result data to write up a PhD thesis?



#14226 pdf split and merge

Posted by Minnie Mouse on 02 February 2009 - 01:54 PM in Be a Geek

Welcome back, Fred_33.

Thank you for the link.



#18452 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 07:33 PM in Venting and Counseling

This is not unexpected -- the PIs responsibilities to a grad student are greater than those to a technician. I'm not being harsh, but it's the truth. Producing a well-trained and successful grad student is more beneficial to a PIs career than is having a successful technician, and failing to produce a successful grad student is worse for the PI. The relationships are totally different, and should be. BTW, I was a lab tech for years, so I know where you're coming from.

You're not obsolete, it's just that your role in the lab has changed.


But aren't it unfair for the technician.
Technician may stay in the lab for >5 years. But PhD student only 3-4 years, master student 2 years.

I used to work in the lab, which values technician more than PhD student.
When the lab is low in budget, the PI would rather cut the PhD's experiment than the technician, because the university did not pay for the training cost for this PhD student.



#18413 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 01:40 PM in Venting and Counseling

Why not get into Graduate School?
To become equal to the student.



#20732 Newborn Bovine Serum

Posted by Minnie Mouse on 01 April 2009 - 04:14 PM in Tissue and Cell Culture

Serum is the product of clotted blood. Blood clots form in response to a cutaneous wound. The growth factors derived from platelet degranulation (primarily PDGF, but also TGF-beta, IGF, FGF, EGF, etc.) are what make your tissue culture cells grow. These factors also drive the early stages of wound healing. Fetal wounds heal more quickly than adult ones, and without scarring. One of the reasons for this is hypothesized to be that the cells activated by fetal serum grow more quickly than those of adults, due to a different balance of cytokines. Thus cells grow more quickly in FCS than in NBCS where they tend to grow bigger and slower.
FCS is the only one of the three that does not contain antibodies.
ECM factors, such as fibronectin, are also present in serum.
-Heat treatment inactivates complement and should always be done with FCS!

These cytokines are not present in BSA.

-tfitzwater-




#16673 New Microbiology Guide

Posted by Minnie Mouse on 23 February 2009 - 01:49 PM in -Microbiology and Virology-

I cannot open the link. :)



#17450 More informative error messages

Posted by Minnie Mouse on 02 March 2009 - 01:23 PM in Suggestion

Please check this thread

http://www.protocol-...?showtopic=6486



#13834 member ratings

Posted by Minnie Mouse on 29 January 2009 - 05:35 PM in Suggestion

Thank you.

I changed it back to "Super Mouse"



#19757 Masters in Microbiology

Posted by Minnie Mouse on 23 March 2009 - 06:49 PM in Career Advice

I am wondering...

Why are you doing master after PhD? :lol:



#20608 male/female ratio

Posted by Minnie Mouse on 31 March 2009 - 01:56 PM in Chit Chat

In medical school, 50% male and 50% female students.

In research Institute, 80% female 20% male.
But in PI level, 80% male and 20% female.




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