We use a "sand bath", an old, leaking water bath with clean, sterilised quartz sand. Not bad for some experiments. And they are also offered new by some companies . I guess sand has better heat transmission compared to beads.
The classic sand bath has some similar qualities to the bead bath, however, the Bath Beads by LabArmor have a superior rate of high efficiency thermal transfer when compared to sand, plus instrument burnout protection, and are mobile and field use compatible.
Have you done a test to compare the bead and the sand bath?
1. Do I have to calculate average or mean? 2. What Test do I have to use to get p values? 3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? standard error(SE) OR standard deviation(SD)?
I think you can treat HL60 cells immediately after seeding.
They don't need to recover from trypsinize nor waiting them to adhere to the bottom of the plate/flask.
As long as you don't spin them too hard before seeding them.
Why do you not try to get a "real" PostDoc position? Or try to get some kind of scholarship for your work. At least here in europe there are several possibilities to get some funding for yourself and your work (e.g. EMBO). But I would never take a loan to get a self funded PostDoc......I think you will be not taken serious in the lab if you do this. And you could get in a bad situation as this could put a lot of pressure on other people in the lab who feel forced to invest their own money into keeping their job.....
Thank you for the link. I may apply for the fellowship.
Because of global recession, it is very difficult to find a "real" job.
My cousin told me that many people are now working unpaid. He had suggested me to look for unpaid job.
Is it good idea to ask for a 3 year self-funded post-doc position? or Ask for 1 year self-funded post-doc postion and willing to pay for the training cost? (I probably need to get a personal loan to pay for it. )
I haven't officially apply for job yet because my current mentor told me that I need to complete 95% of the PhD thesis before applying for job.
In the past few months, I am just asking around to see whether PI will take me as a self-funded post-doc. In other words, bypassing my current mentor. But I haven't been successful in securing a self-funded post-doc position for 1 year. I can only get a 3 months volunteer technician.
mdfenko had mentioned the reason why PI may not want a self-funded post-doc for 1 year... that is why I am thinking of doing self-funded post-doc for 3 year.
yes, i think the recession affects jobs in research. pharma is laying off and grants are more difficult to obtain. government jobs are frozen or being reduced. schools are getting reduced funding.
although you would think that any pi would take a self-funded postdoc you have to realize that there are other expenses than salary. supplies are required, with a certain amount of expected waste, when training someone. the pis may think that one year is not worth the investment and they can't afford to extend your stay.