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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 20-January 19)



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#14662 Cell count using a hemocytometer

Posted by Minnie Mouse on 05 February 2009 - 03:28 PM in Tissue and Cell Culture

Cell count using a hemocytometer

http://www.abcam.com...g...e&rid=11454



#14638 Buffers that are good for cell culture.

Posted by Minnie Mouse on 05 February 2009 - 01:33 PM in Tissue and Cell Culture

What cell line are you growing?



#14503 BioForum trawling

Posted by Minnie Mouse on 04 February 2009 - 01:00 PM in Chit Chat

There is a "Search" buttom at the top right hand corner and a "Forum Archives" buttom at the top...slightly to the right.

Hope this may help.



#14268 BioForum Archives (1999-2009) are available for browsing and searching

Posted by Minnie Mouse on 02 February 2009 - 08:29 PM in News and Announcements

Thank you.

Luckily, we haven't lost everything. :P



#14265 Best storage temperature for samples - serum, protein, tissue, DNA, RNA

Posted by Minnie Mouse on 02 February 2009 - 08:20 PM in General Lab Techniques

Due to spatial limitations in our lab freezers i want to reorganize my samples. can anyone of you tell me where different kinds of samples should be longtime stored?

-20°C, -80°C, -196°C...

Serum
Tissue
Proteinextracts
RNA
DNA
Bacterial Strains
Tissue Sections on Slides
...

Thanks for recommendations!

-moljul-
________________________________________________________________________________
_____________

Why don't you just keep them at the same temps. where they are stored right now. Even if you reorganize you shouldn't put them into different freezers. Sounds like an exam question to me ;-)


I help you anyway:
- DNA: Plasmids at -20C, Genomic at 4C. DNA can fall out of solution if stored at -20C. With plasmids that is no problem because you can vortex them and get them back into solution. Genomic DNA is dangerous to vortex because you might break the DNA
- RNA: Is less stable than DNA so we store it at -80. But you should be able to store it at -20C too
- Bacteria: -80C
- Serum: -20C
- Protein: - 20C
- Tissue: Depends. Ours are formalin fixed so we leave them in the fridge. Flash frozen samples in liquid nitrogen

-UGA80-
________________________________________________________________________________
_____________

-4`C -- Tissue section on slides
-20`C -- Serum, DNA, RNA, protein extracts
-80`C -- Bacterial strains (in LB),
-196`C -- Tissue

-tzyyyue-



#31119 Beginner Question

Posted by Minnie Mouse on 30 July 2009 - 04:39 PM in SDS-PAGE and Western Blotting

May I ask what protein expression are you looking for?



#20615 B*%$%y government! Nanny state gone too far

Posted by Minnie Mouse on 31 March 2009 - 03:21 PM in Venting and Counseling

or develop a nut vaccine



#21754 automated protein structure search

Posted by Minnie Mouse on 13 April 2009 - 09:54 PM in Bioinformatics and Biostatistics

BLAST

http://blast.ncbi.nl...h.gov/Blast.cgi



#14138 Apoptosis Protocol

Posted by Minnie Mouse on 01 February 2009 - 02:36 PM in Apoptosis, Necrosis and Autophagy

Introduction to apoptosis
http://www.abcam.com...g...e&rid=11367

Annexin V detection protocol
http://www.abcam.com...g...e&rid=11369

Apoptosis induction protocol
http://www.abcam.com...g...e&rid=11368

Caspase detection protocol
http://www.abcam.com...g...e&rid=11370

DNA fragmentation analysis protocol
http://www.abcam.com...g...e&rid=11371



#13838 all gone??????

Posted by Minnie Mouse on 29 January 2009 - 05:59 PM in Chit Chat

research gate is over, but has anybody visited this molecular station site???? It seems there are some vultures around....... :wacko:

do U remember there was another dude asking for name for a scientist facebook. I wonder what happened to him.


But some are lost, for example toejam, vetticus3, fred33, aimikins (?). Have a poor memory for (nick-)names.


Rhombus, Calvin*, cellcounter, and phage434 may be lost.



#17756 A manual of techniques for separation, detection and recovery of nucleic acids a

Posted by Minnie Mouse on 04 March 2009 - 02:04 PM in Free Stuff

Thank you lactolee




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