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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 21-September 19)



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#13824 Will the global recession affect the job market for research?

Posted by Minnie Mouse on 29 January 2009 - 03:46 PM in Career Advice

Do you think the global recession will affect the availbility of job in research?

I will be submitting my PhD thesis after three months.
At the moment, I can only find a three month volunteer technician job, which I can start after submitting my thesis.

I have been asking around but most PI aren't interest in taking a post-doc, not even a self-funded post-doc. :P

Any tips in persuading people to take me as a self-funded post-doc?
I don't need the PI to pay me a salary, as I have enough saving to support myself for one year.
As long as the PI is willing to train me...not just making buffer and filling tip-box.

Minnie is very desperate for a job. I know many people are now unemploy.
It would be difficult for me to find a job. :wacko:



#14231 Will the global recession affect the job market for research?

Posted by Minnie Mouse on 02 February 2009 - 02:22 PM in Career Advice

Funding will be reduced in research due to global recession. ;)

Will there be massive layoff in research? ;)



#18413 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 01:40 PM in Venting and Counseling

Why not get into Graduate School?
To become equal to the student.



#18452 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 07:33 PM in Venting and Counseling

This is not unexpected -- the PIs responsibilities to a grad student are greater than those to a technician. I'm not being harsh, but it's the truth. Producing a well-trained and successful grad student is more beneficial to a PIs career than is having a successful technician, and failing to produce a successful grad student is worse for the PI. The relationships are totally different, and should be. BTW, I was a lab tech for years, so I know where you're coming from.

You're not obsolete, it's just that your role in the lab has changed.


But aren't it unfair for the technician.
Technician may stay in the lab for >5 years. But PhD student only 3-4 years, master student 2 years.

I used to work in the lab, which values technician more than PhD student.
When the lab is low in budget, the PI would rather cut the PhD's experiment than the technician, because the university did not pay for the training cost for this PhD student.



#20615 B*%$%y government! Nanny state gone too far

Posted by Minnie Mouse on 31 March 2009 - 03:21 PM in Venting and Counseling

or develop a nut vaccine



#22157 Waterless Water Baths?

Posted by Minnie Mouse on 17 April 2009 - 04:52 PM in Cell Biology Products

We use a "sand bath", an old, leaking water bath with clean, sterilised quartz sand. Not bad for some experiments.
And they are also offered new by some companies . I guess sand has better heat transmission compared to beads.



The classic sand bath has some similar qualities to the bead bath, however, the Bath Beads by LabArmor have a superior rate of high efficiency thermal transfer when compared to sand, plus instrument burnout protection, and are mobile and field use compatible.

DW


Have you done a test to compare the bead and the sand bath?



#17589 rating

Posted by Minnie Mouse on 03 March 2009 - 01:25 PM in Chit Chat

I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)


It is back now.


Thank you, Bioforum



#17474 rating

Posted by Minnie Mouse on 02 March 2009 - 06:55 PM in Chit Chat

This feature was in the old forum, I'm quite sure of that. The geek forum has it (you can rate all the topics over there) and it's new. But for the tech forums, some subforums have and some don't, so it's really intriguing. And at least now we know that moderators are not culpable, there go our escape goats :D ....


I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)



#17473 rating

Posted by Minnie Mouse on 02 March 2009 - 06:48 PM in Chit Chat

lol......the same topic that you replied earlier had rating....the Taqman primer and probe.


I would like to know how many posts I'd have to get PM enabled. I was in the middle of giving advice to someone and waiting for the answer, oops.
And I am not such a fervent poster to quickly make the necessary amount.

EDIT: oooh it works.


I think PM is enabled when you reach "two blue squares".



#14366 Shameless Advertising

Posted by Minnie Mouse on 03 February 2009 - 06:07 PM in Chit Chat

Don't worry. I also got one.

I signed up at Bioforum in around July 2005, and re-registered after the crash.



#31647 Happy Birthday Mdfenko

Posted by Minnie Mouse on 04 August 2009 - 03:51 AM in Chit Chat

Happy belated Birthday. :(



#27237 Inconsistent ELISA results

Posted by Minnie Mouse on 21 June 2009 - 07:45 PM in ELISA and Immunoassay

you may try to

1. Calibrate the ELISA plate reader

2. use a very good multichannel pipette

I hope this may help.



#21610 happy birthday swanny!

Posted by Minnie Mouse on 12 April 2009 - 04:04 PM in Chit Chat

Happy Birthday, swanny.

Foster is a brand of beer.



#13849 Handbook of Biological Statistics

Posted by Minnie Mouse on 29 January 2009 - 07:05 PM in Bioinformatics and Biostatistics

Thank you, gebirgsziege

This thread is pinned.



#28461 It's TJ's B-day

Posted by Minnie Mouse on 06 July 2009 - 07:09 PM in Chit Chat

Happy Birthday, toejam :)

Hope you receive many presents.



#13811 tree fall

Posted by Minnie Mouse on 29 January 2009 - 01:50 PM in Lab Jokes

:D :wacko: :D :P



#19749 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 03:57 PM in Flow Cytometry

This e-book is >74MB. It took me a long time to download.



#13520 Fluorescence & Flow Cytometry Tutorial

Posted by Minnie Mouse on 27 January 2009 - 01:41 PM in Flow Cytometry

Fluorescence & Flow Cytometry Tutorial

http://www.invitroge.../Tutorials.html



#13814 Some useful resources for writing

Posted by Minnie Mouse on 29 January 2009 - 02:03 PM in Dissertation and Paper Writing

thank you



#17063 Seeding volume in cell culture flasks

Posted by Minnie Mouse on 26 February 2009 - 01:41 PM in Cell Biology

In my hands, no.

The reason is when you lay the flask on its side during incubation, the amount of media flows too closely to the neck which could lead to contamination.

For suspension cultures, the largest volume I've used is 16 mL.
If you need larger volume, try a spinner flask or roller bottle.



yes your are correct i used only 15ml but i saw that we can make upto 40ml
i want to try...as it is not possible to keep the flask flat ...could it be possible to stand the flask ?
do the cells still be happy in that conditions with less length?


I don't think your cell will be happy.
If you stand the flask, the cell will accumulate at the bottom of the flask and reduce the area for them to grow.
The cells at the very bottom will not get nutrient from the medium. Unless you gently shake the flask every hour.

This is why people invented rolling bottle.

Hope this may help.



#13517 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 27 January 2009 - 01:37 PM in Flow Cytometry

Practical Flow Cytometry, Fourth Edition


http://www.invitroge...-Cytometry.html



#13820 Some useful resources for writing

Posted by Minnie Mouse on 29 January 2009 - 02:31 PM in Dissertation and Paper Writing

How to write a good scientific paper.


Attached File  How_to_write_a_good_scientific_paper.pdf   274.89KB   1041 downloads



#19734 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 01:54 PM in Flow Cytometry

Thank you, yobou



#14504 What is the rank of assistant professor?

Posted by Minnie Mouse on 04 February 2009 - 01:15 PM in Career Advice

Do you have >1 year of teaching experience?



#14873 How to use Google search to help your writing

Posted by Minnie Mouse on 07 February 2009 - 02:59 PM in Dissertation and Paper Writing

Thank you pcrman.

I did't know Google is so useful in writing.

This thread is pinned.




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