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Minnie Mouse's Content
There have been 136 items by Minnie Mouse (Search limited from 21-September 19)
I will be submitting my PhD thesis after three months.
At the moment, I can only find a three month volunteer technician job, which I can start after submitting my thesis.
I have been asking around but most PI aren't interest in taking a post-doc, not even a self-funded post-doc.
Any tips in persuading people to take me as a self-funded post-doc?
I don't need the PI to pay me a salary, as I have enough saving to support myself for one year.
As long as the PI is willing to train me...not just making buffer and filling tip-box.
Minnie is very desperate for a job. I know many people are now unemploy.
It would be difficult for me to find a job.
This is not unexpected -- the PIs responsibilities to a grad student are greater than those to a technician. I'm not being harsh, but it's the truth. Producing a well-trained and successful grad student is more beneficial to a PIs career than is having a successful technician, and failing to produce a successful grad student is worse for the PI. The relationships are totally different, and should be. BTW, I was a lab tech for years, so I know where you're coming from.
You're not obsolete, it's just that your role in the lab has changed.
But aren't it unfair for the technician.
Technician may stay in the lab for >5 years. But PhD student only 3-4 years, master student 2 years.
I used to work in the lab, which values technician more than PhD student.
When the lab is low in budget, the PI would rather cut the PhD's experiment than the technician, because the university did not pay for the training cost for this PhD student.
We use a "sand bath", an old, leaking water bath with clean, sterilised quartz sand. Not bad for some experiments.
And they are also offered new by some companies . I guess sand has better heat transmission compared to beads.
The classic sand bath has some similar qualities to the bead bath, however, the Bath Beads by LabArmor have a superior rate of high efficiency thermal transfer when compared to sand, plus instrument burnout protection, and are mobile and field use compatible.
Have you done a test to compare the bead and the sand bath?
This feature was in the old forum, I'm quite sure of that. The geek forum has it (you can rate all the topics over there) and it's new. But for the tech forums, some subforums have and some don't, so it's really intriguing. And at least now we know that moderators are not culpable, there go our escape goats ....
I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum
lol......the same topic that you replied earlier had rating....the Taqman primer and probe.
I would like to know how many posts I'd have to get PM enabled. I was in the middle of giving advice to someone and waiting for the answer, oops.
And I am not such a fervent poster to quickly make the necessary amount.
EDIT: oooh it works.
I think PM is enabled when you reach "two blue squares".
In my hands, no.
The reason is when you lay the flask on its side during incubation, the amount of media flows too closely to the neck which could lead to contamination.
For suspension cultures, the largest volume I've used is 16 mL.
If you need larger volume, try a spinner flask or roller bottle.
yes your are correct i used only 15ml but i saw that we can make upto 40ml
i want to try...as it is not possible to keep the flask flat ...could it be possible to stand the flask ?
do the cells still be happy in that conditions with less length?
I don't think your cell will be happy.
If you stand the flask, the cell will accumulate at the bottom of the flask and reduce the area for them to grow.
The cells at the very bottom will not get nutrient from the medium. Unless you gently shake the flask every hour.
This is why people invented rolling bottle.
Hope this may help.