Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 22-November 18)



Sort by                Order  

#21610 happy birthday swanny!

Posted by Minnie Mouse on 12 April 2009 - 04:04 PM in Chit Chat

Happy Birthday, swanny.

Foster is a brand of beer.



#21460 confusion about 5'UTR

Posted by Minnie Mouse on 09 April 2009 - 09:44 PM in Molecular Cloning

Hi Chicken,

Easter is coming.
Some people are on holiday or are busy finishing the experiments before the holiday.

After Easter Holiday, there will be more people to answer the posts.



#21456 male/female ratio

Posted by Minnie Mouse on 09 April 2009 - 08:54 PM in Chit Chat

There is a spy amongst us or someone seriously read this thread

Univ of Tokyo to increase number of female researchers, ban evening meetings


Meeting starts after 5pm in University of Tokyo!! ;) Then, when do their meetings end?...7 - 8pm!! :blink:

I never have lab meeting that starts after 5pm. I have meeting that ends after 5pm.



#21449 How many of you doing research as a passion not for survial

Posted by Minnie Mouse on 09 April 2009 - 07:56 PM in Chit Chat

How many of you really are doing research as a passion instead of for survival, and really believe what you are doing is going to solve some real problem. I believe over 90% of us will respond "no" including myself.


me, yes ;)

I worked as a volunteer technician for 6 months, then worked as a fully self-funded PhD student for 2 years in the same lab.

If it is not for passion, I would do a MBA instead...
A small investment company offered to pay for my MBA tuition and gave me a brand new BMW if I do MBA and continue working in their company.



#21439 male/female ratio

Posted by Minnie Mouse on 09 April 2009 - 03:48 PM in Chit Chat

I have seen all female in a large lab (>8 people), but I never see all male in a large lab.



#21226 Extended Essay Suggestions..?

Posted by Minnie Mouse on 07 April 2009 - 01:24 PM in Research Idea, Design and Collaboration

Which area of biology interest you?
Zoology, Botany, biochemistry, microbiology, genetics



#21159 Who is back?

Posted by Minnie Mouse on 06 April 2009 - 06:03 PM in Chit Chat

Uncle Rhombus is back :D

Here are his recent posts.
http://www.protocol-...sult_type=posts

I miss his brain :) - 30 years of tissue culture experience. :rolleyes:



#21150 Extended Essay Suggestions..?

Posted by Minnie Mouse on 06 April 2009 - 03:44 PM in Research Idea, Design and Collaboration

Are you going to do small experiment?



#21145 male/female ratio

Posted by Minnie Mouse on 06 April 2009 - 02:21 PM in Chit Chat

Someone said that a tall strong male has more advantage in applying for a job in a lab than a short weak female, such as minnie. :rolleyes:



#20839 Trained

Posted by Minnie Mouse on 02 April 2009 - 01:16 PM in Lab Jokes

This sounds like my dog.

He sits beside his snack box patiently and we give him a snack.

Sometimes, he would sit for half an hour.

(minnie) mouse has a dog? U meant Pluto?

Posted Image


yes
DSCN0702.JPG



#20837 Happy Birthday, aimikins

Posted by Minnie Mouse on 02 April 2009 - 01:10 PM in Chit Chat

Happy Birthday, aimikins. :lol:

Hope you receive many presents from Calvin. :D



#20836 Liquid and solid autoclave

Posted by Minnie Mouse on 02 April 2009 - 01:07 PM in General Lab Techniques

I would autoclave solid (tips boxes and eppendorf) and liquid (buffers) together using liquid cycle, rather than separate them and run two different cycles.

It would help to reduce greenhouse gases.



#20732 Newborn Bovine Serum

Posted by Minnie Mouse on 01 April 2009 - 04:14 PM in Tissue and Cell Culture

Serum is the product of clotted blood. Blood clots form in response to a cutaneous wound. The growth factors derived from platelet degranulation (primarily PDGF, but also TGF-beta, IGF, FGF, EGF, etc.) are what make your tissue culture cells grow. These factors also drive the early stages of wound healing. Fetal wounds heal more quickly than adult ones, and without scarring. One of the reasons for this is hypothesized to be that the cells activated by fetal serum grow more quickly than those of adults, due to a different balance of cytokines. Thus cells grow more quickly in FCS than in NBCS where they tend to grow bigger and slower.
FCS is the only one of the three that does not contain antibodies.
ECM factors, such as fibronectin, are also present in serum.
-Heat treatment inactivates complement and should always be done with FCS!

These cytokines are not present in BSA.

-tfitzwater-




#20627 male/female ratio

Posted by Minnie Mouse on 31 March 2009 - 07:04 PM in Chit Chat

9M : 3 F



at work or at school?

It seems strange but at work there are more men then women (research or production or even teaching) here while at university there are more women in the specific domain.

Sorry. Wrote that in hurry.
(New Data)
1F-Lab Manager and Research Assoc(technician), 1F Research Assoc (tech), 1M Prof (MD-PhD), 4M (MD-PhD), 1F (MD-PhD Student), 6M (MD-PhD student)


Are you one of the MD-PhD student?



#20615 B*%$%y government! Nanny state gone too far

Posted by Minnie Mouse on 31 March 2009 - 03:21 PM in Venting and Counseling

or develop a nut vaccine



#20614 Liquid and solid autoclave

Posted by Minnie Mouse on 31 March 2009 - 02:59 PM in General Lab Techniques

Normally, I autoclave buffer and tip boxes together using liquid cycle.



#20613 self-funded post-doc position

Posted by Minnie Mouse on 31 March 2009 - 02:54 PM in Career Advice

Why do you not try to get a "real" PostDoc position? Or try to get some kind of scholarship for your work. At least here in europe there are several possibilities to get some funding for yourself and your work (e.g. EMBO).
But I would never take a loan to get a self funded PostDoc......I think you will be not taken serious in the lab if you do this. And you could get in a bad situation as this could put a lot of pressure on other people in the lab who feel forced to invest their own money into keeping their job.....


Thank you for the link. I may apply for the fellowship.

Because of global recession, it is very difficult to find a "real" job.

My cousin told me that many people are now working unpaid.
He had suggested me to look for unpaid job.



#20610 Who is back?

Posted by Minnie Mouse on 31 March 2009 - 02:07 PM in Chit Chat

I too am back.

I wandered off for a month or so and came back and had to re-register. My lovely post-count gone forever!

Imagine how fred_33 feels!


Why, how many posts did he had?


fred_33 had >3000 posts

I had only >1000 posts.



#20608 male/female ratio

Posted by Minnie Mouse on 31 March 2009 - 01:56 PM in Chit Chat

In medical school, 50% male and 50% female students.

In research Institute, 80% female 20% male.
But in PI level, 80% male and 20% female.



#19991 self-funded post-doc position

Posted by Minnie Mouse on 25 March 2009 - 03:28 PM in Career Advice

Is it good idea to ask for a 3 year self-funded post-doc position?
or
Ask for 1 year self-funded post-doc postion and willing to pay for the training cost?
(I probably need to get a personal loan to pay for it. )

I haven't officially apply for job yet because my current mentor told me that I need to complete 95% of the PhD thesis before applying for job.

In the past few months, I am just asking around to see whether PI will take me as a self-funded post-doc.
In other words, bypassing my current mentor. :P
But I haven't been successful in securing a self-funded post-doc position for 1 year. :lol:
I can only get a 3 months volunteer technician. :lol:

mdfenko had mentioned the reason why PI may not want a self-funded post-doc for 1 year... that is why I am thinking of doing self-funded post-doc for 3 year.

yes, i think the recession affects jobs in research. pharma is laying off and grants are more difficult to obtain. government jobs are frozen or being reduced. schools are getting reduced funding.

although you would think that any pi would take a self-funded postdoc you have to realize that there are other expenses than salary. supplies are required, with a certain amount of expected waste, when training someone. the pis may think that one year is not worth the investment and they can't afford to extend your stay.




#19988 Statistics help

Posted by Minnie Mouse on 25 March 2009 - 02:30 PM in Bioinformatics and Biostatistics

two-way ANOVA



#19868 Who have we lost?

Posted by Minnie Mouse on 24 March 2009 - 01:34 PM in Chit Chat

hello all :)
it is sooooo nice to re-join again :)

where is krumelmonester ??? :)
- if this is how it is spelled :(-


Where is the cookie monster? :)



#19757 Masters in Microbiology

Posted by Minnie Mouse on 23 March 2009 - 06:49 PM in Career Advice

I am wondering...

Why are you doing master after PhD? :lol:



#19749 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 03:57 PM in Flow Cytometry

This e-book is >74MB. It took me a long time to download.



#19743 We're all wasting our time!!!

Posted by Minnie Mouse on 23 March 2009 - 02:46 PM in Lab Jokes

I also got a paper. :lol: :D

But I cannot open it? Only get an error message.


It is now fixed. :)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.