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Oracle602's Content

There have been 3 items by Oracle602 (Search limited from 28-May 19)


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#9585 Transformation Efficiency

Posted by Oracle602 on 26 December 2004 - 10:17 AM in Molecular Biology

E.coli
Trying to incorporate various environmental resistance genes



#9580 I'm lost... What to do after PhD???

Posted by Oracle602 on 25 December 2004 - 07:08 PM in Career Advice

In my personal opinion, whatever you do, don't give up! Finish the job, do the research. If I were to drop what you are doing I would feel that those years of life would be a waste. When you're done, you can use your knowledge to incorporate things you like with the field you specialize in. (Doctor hating medicine and loving computers made a clinical application program that all doctors use).
But then again, I"m not speaking from experience. Hopefully some members of this board can give their experienced opinion.

Good luck to you!

BigO



#9579 Transformation Efficiency

Posted by Oracle602 on 25 December 2004 - 06:59 PM in Molecular Biology

What factors would you say could affect the transformation efficiency in incorporating supercoiled plasmid DNA into a unicellular eukaryote?
I used the calcium chloride protocol. Temperature of incubation? heat shock? amounts of cells/plasmid used?

Thanks in Advance,
bigO




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