I also grow them in RPMI-1640 with 10% FBS, they grow well, the doubling time is silimar to ATCC reported. but when I set up the 96-well plate, treat with various doses of estradiol, they didn'grow well, and there are more and more dead cells,I have no idea what happened to these cells, Dose anyone have any idea? I repeated twice, same thing happened.Can anyone tell me how you do the experiment of MCF-7 cells treated with 17-beta estradiol?
I grow MCF-7 in DMEM+10% FBS+ antibiotic and they are growing just fine.. they are kind of slow.. so what I do is increase the number of cells, i usually seed around 0.7 million cells in p-25 and they grow to full confulency within a week. you just control the number of cells seeded.
I never change the media the next day, usually do that the third day. and I never added insuline.. and they are growing just fine... and mine grow in epithelila layer form not doms
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